Berkeley Lab's Environmental Genomics & Systems BiologyDivision has an opening for a Postdoctoral Researcher. Bacterial microcompartments are organelles composed entirely of protein that enable bacteria to use diverse substrates in nutritionally poor environments, thereby contributing to the fitness of the pathogens in host tissues. They are composed of a selectively permeable protein shell that encapsulates catabolic enzymes. The shell is the defining feature of all types of bacterial microcompartments, providing the diffusion barrier that typically enables concentration of substrates in the vicinity of the encapsulated enzymes, sequesters toxic intermediates or protects oxygen sensitive enzymes from the bulk cytosol. How these shells, composed of multiple types of paralogs, self-assemble and the structural basis of the selective permeability of a protein shell are fundamental biological questions with important practical implications; the project focuses on disrupting shell assembly and permeability to provide a novel strategy for the design of anti-microbials.
The Postdoctoral Researcher will carry out creative research with tenacity and of the highest quality standards. This includes experimental design, trouble shooting, interpretation of data and the ability to situate results in the context of disarming pathogens by disabling BMC-based metabolism. Research will involve protein/enzyme biochemistry, functional and structural characterization (including EM techniques, x-ray crystallography, x-ray footprinting), high-throughput screening of small molecule/peptide binding and synthetic biology-based approaches in vitro and in E. coli. The goal is to identify factors that preclude shell formation or alter permeability, with the long-term objective of using the findings in the development of therapeutics. Closely related projects are at various experimental stages, The Postdoc willcollaborate with other group members on a range of fundamental and applied studies of BMCs.
What You Will Do:
Carry out creative research with tenacity and of the highest quality standards. This includes experimental design, trouble shooting, interpretation of data and the ability to situate results in the context of disarming pathogens by disabling BMC-based metabolism
Develop and execute small molecule and peptide based screens in a high throughput format
Prepare manuscripts for publication and assist with the development of funding proposals and patents
Overexpression and purification and characterization of proteins/enzymes
Genetic manipulation of microorganisms
Conduct and perform collaborative research and effectively interact with a broad range of colleagues
Organize and present reports to collaborators and sponsors.
Additional Responsibilities as needed:
Participate in professional scientific activities, e.g., reviews of papers, research proposals, etc.
Support and contribute to the development of research project ideas and proposals.
Participate in relevant professional societies with relevance to the program and group activities.
What is Required:
PhD in biochemistry, molecular biology, biophysics or related field.
Experience in one or more of the following: synthetic biology or bioengineering, protein design, high-throughput approaches to cloning and imaging, enzymology or biophysical characterization of macromolecular complexes.
Strong background in molecular biology and/or biochemistry who can interact collaboratively among a variety of disciplines.
Basic working knowledge-based use of bioinformatics tools.
Additional Desired Qualifications:
Experience establishing high-throughput assays
Experience in cellular imaging, bioinformatics or enzyme characterization preferred.
The posting shall remain open until the position is filled.
This is a full time, 1 year, postdoctoral appointment with the possibility of renewal based upon satisfactory job performance, continuing availability of funds and ongoing operational needs. You must have less than years paid postdoctoral experience. Salary for Postdoctoral positions depends on years of experience post-degree.
Full-time, M-F, exempt (monthly paid) from overtime pay.
This position is represented by a union for collective bargaining purposes.
Salary will be predetermined based on postdoctoral step rates.
This position may be subject to a background check. Any convictions will be evaluated to determine if they directly relate to the responsibilities and requirements of the position. Having a conviction history will not automatically disqualify an applicant from being considered for employment.
Work will be primarily performed at Lawrence Berkeley National Lab, 1 Cyclotron Road, Berkeley, CA.
Berkeley Lab (LBNL) addresses the world's most urgent scientific challenges by advancing sustainable energy, protecting human health, creating new materials, and revealing the origin and fate of the universe. Founded in 1931, Berkeley Lab's scientific expertise has been recognized with 13 Nobel prizes. The University of California manages Berkeley Lab for the U.S. Department of Energy's Office of Science.
Equal Employment Opportunity: Berkeley Lab is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, or protected veteran status. Berkeley Lab is in compliance with the Pay Transparency Nondiscrimination Provision under 41 CFR 60-1.4. Click here to view the poster and supplement: "Equal Employment Opportunity is the Law."
Internal Number: 87235
About Lawrence Berkeley National Laboratory
In the world of science, Lawrence Berkeley National Laboratory (Berkeley Lab) is synonymous with excellence. Thirteen scientists associated with Berkeley Lab have won the Nobel Prize. Fifty-seven Lab scientists are members of the National Academy of Sciences (NAS), one of the highest honors for a scientist in the United States. Thirteen of our scientists have won the National Medal of Science, our nation's highest award for lifetime achievement in fields of scientific research. Eighteen of our engineers have been elected to the National Academy of Engineering, and three of our scientists have been elected into the Institute of Medicine. In addition, Berkeley Lab has trained thousands of university science and engineering students who are advancing technological innovations across the nation and around the world. Berkeley Lab is a member of the national laboratory system supported by the U.S. Department of Energy through its Office of Science. It is managed by the University of California (UC) and is charged with conducting unclassified research across a wide range of scientific disciplines. Located on a 200-acre site in the hills above the UC Berkeley campus that offers spectacular... views of the San Francisco Bay, Berkeley Lab employs approximately 4,200 scientists, engineers, support staff and students. Its budget for 2011 is $735 million, with an additional $101 million in funding from the American Recovery and Reinvestment Act, for a total of $836 million. A recent study estimates the Laboratory's overall economic impact through direct, indirect and induced spending on the nine counties that make up the San Francisco Bay Area to be nearly $700 million annually. The Lab was also responsible for creating 5,600 jobs locally and 12,000 nationally. The overall economic impact on the national economy is estimated at $1.6 billion a year. Technologies developed at Berkeley Lab have generated billions of dollars in revenues, and thousands of jobs. Savings as a result of Berkeley Lab developments in lighting and windows, and other energy-efficient technologies, have also been in the billions of dollars. Berkeley Lab was founded in 1931 by Ernest Orlando Lawrence, a UC Berkeley physicist who won the 1939 Nobel Prize in physics for his invention of the cyclotron, a circular particle accelerator that opened the door to high-energy physics. It was Lawrence's belief that scientific research is best done through teams of individuals with different fields of expertise, working together. His teamwork concept is a Berkeley Lab legacy that continues today.