Berkeley Lab's Biological Systems & Engineering Division has an opening for a Postdoctoral Scholar. The Biological Systems & Engineering Division of Berkeley Lab has an exciting opening in the Bioengineering and Biomedical Sciences Department for a Biochemistry Postdoctoral Scholar to join a team that focuses on elucidating the molecular mechanisms that underlie aging and their relationship to genome instability and cancer. The project is part of a large multi-institutional Program funded by the National Institute on Aging of NIH. Under the general direction of the P.I., the Postdoc will conduct independent basic research in molecular and cell biology and biochemistry and will use human and rodent cell systems to address relationships between DNA repair, genome stability, cancer and aging, particularly with regard to the roles of multi-component protein complexes. The project involves close collaborations with The Buck Institute for Research on Aging (Novato, CA) and the Erasmus Medical Center (Rotterdam).
Aging is intimately intertwined with genome maintenance. Replication and transcription impose special demands, requiring the coordinated contribution of multiple DNA repair processes to maintain genomic integrity. Breakdown in any of these systems or in their coordination can cause replication fork collapse and DNA double-strand breaks (DSBs), transcriptional stress, and genomic instability – a hallmark of aging. The Postdoc will join a project that is designed to provide molecular mechanistic understanding of how particular key genome maintenance proteins prevent aging pathologies. Research will be conducted to test the overarching hypothesis that integration and coordination of multiple DNA repair processes with each other and with the replication and transcription machineries maintains damage-free templates for transcription with avoidance of transcriptional stress, which is proposed to be a novel major contributor to aging.
What You Will Do:
Design and construct mammalian expression vectors for recombinant proteins and reporter constructs; transfect into human or mouse cells for analysis of functional endpoints.
Knockdown selected proteins in mammalian cells using siRNA or shRNA; mutate and/or tag genomic copies of mammalian DNA repair genes in situ using CRISPR/Cas9 technologies.
Investigate and characterize protein-protein interactions and regulation of protein function by assembly into complexes and/or by post-translational modifications.
Use ChIP-qPCR to analyze recruitment of specific proteins to defined DSBs in particular genomic contexts using a novel human cell system and assay repair kinetics of these DSBs after knockdown of specific proteins.
Characterize the effects of alterations in key DNA repair proteins on cell growth, viability, transcriptional stress, R-loop formation, and cellular responses to DNA damage, especially cell fate outcomes (cellular senescence, the senescence-associated secretory phenotype, and induction of apoptosis).
Analyze data and prepare results for presentation; present research data at group meetings and at conferences as appropriate.
Prepare manuscripts for publication in peer-reviewed scientific journals.
Maintain an accurate and detailed laboratory notebook of all experiments performed. Provide sufficient information to ensure that the work could be reproduced
Interact productively with other members of the P.I.'s laboratory and with collaborating laboratories to further the scientific goals of the research program.
Additional Responsibilities as needed:
Participate in group meetings, seminars, and workshops.
Provide guidance to undergraduate students.
Receive training in, and assist the PI with, preparation of grant applications and progress reports.
What is Required:
Ph.D. within the last four years in molecular biology, biochemistry, genetics, or related field.
Experience in protein biochemistry including quantitative western blot analysis, biochemical fractionation of cells, protein-protein and protein-DNA interaction studies, and a range of enzymatic assays.
Experience in ChIP and qPCR analysis.
Extensive experience in mammalian cell culture, transfection, cell biological assays including immunofluorescence, and reporter assays.
Demonstrated ability to design and carry out independent research, with track record of publishing in the peer-reviewed scientific literature.
Strong organizational skills.
Well-developed interpersonal and communication skills, with proven ability to communicate effectively both orally and in writing.
Demonstrated ability to work under PI guidance and with collaborators and to interact effectively with other technical and scientific staff members of a research team, as well as to conduct experiments independently.
Additional Desired Qualifications:
Background and previous research experience in molecular mechanisms of DNA repair and maintenance of genome stability.
Previous research experience in effects of DNA damage on transcription and/or replication.
Background and interest in cellular senescence and aging.
The posting shall remain open until the position is filled.
This is a full time, 1 year, postdoctoral appointment with the possibility of renewal based upon satisfactory job performance, continuing availability of funds and ongoing operational needs. You must have less than 4 years paid postdoctoral experience. Salary for Postdoctoral positions depends on years of experience post-degree.
Full-time, M-F, exempt (monthly paid) from overtime pay.
This position is represented by a union for collective bargaining purposes.
Salary will be predetermined based on postdoctoral step rates.
This position may be subject to a background check. Any convictions will be evaluated to determine if they directly relate to the responsibilities and requirements of the position. Having a conviction history will not automatically disqualify an applicant from being considered for employment.
Work will be primarily performed at West Berkeley Biocenter (Potter St.) — Bldg. 977, 717 Potter St., Berkeley, CA.
Berkeley Lab (LBNL) addresses the world's most urgent scientific challenges by advancing sustainable energy, protecting human health, creating new materials, and revealing the origin and fate of the universe. Founded in 1931, Berkeley Lab's scientific expertise has been recognized with 13 Nobel prizes. The University of California manages Berkeley Lab for the U.S. Department of Energy's Office of Science.
Equal Employment Opportunity: Berkeley Lab is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, or protected veteran status. Berkeley Lab is in compliance with the Pay Transparency Nondiscrimination Provision under 41 CFR 60-1.4. Click here to view the poster and supplement: "Equal Employment Opportunity is the Law."
Internal Number: 85812
About Lawrence Berkeley National Laboratory
In the world of science, Lawrence Berkeley National Laboratory (Berkeley Lab) is synonymous with excellence. Thirteen scientists associated with Berkeley Lab have won the Nobel Prize. Fifty-seven Lab scientists are members of the National Academy of Sciences (NAS), one of the highest honors for a scientist in the United States. Thirteen of our scientists have won the National Medal of Science, our nation's highest award for lifetime achievement in fields of scientific research. Eighteen of our engineers have been elected to the National Academy of Engineering, and three of our scientists have been elected into the Institute of Medicine. In addition, Berkeley Lab has trained thousands of university science and engineering students who are advancing technological innovations across the nation and around the world. Berkeley Lab is a member of the national laboratory system supported by the U.S. Department of Energy through its Office of Science. It is managed by the University of California (UC) and is charged with conducting unclassified research across a wide range of scientific disciplines. Located on a 200-acre site in the hills above the UC Berkeley campus that offers spectacular... views of the San Francisco Bay, Berkeley Lab employs approximately 4,200 scientists, engineers, support staff and students. Its budget for 2011 is $735 million, with an additional $101 million in funding from the American Recovery and Reinvestment Act, for a total of $836 million. A recent study estimates the Laboratory's overall economic impact through direct, indirect and induced spending on the nine counties that make up the San Francisco Bay Area to be nearly $700 million annually. The Lab was also responsible for creating 5,600 jobs locally and 12,000 nationally. The overall economic impact on the national economy is estimated at $1.6 billion a year. Technologies developed at Berkeley Lab have generated billions of dollars in revenues, and thousands of jobs. Savings as a result of Berkeley Lab developments in lighting and windows, and other energy-efficient technologies, have also been in the billions of dollars. Berkeley Lab was founded in 1931 by Ernest Orlando Lawrence, a UC Berkeley physicist who won the 1939 Nobel Prize in physics for his invention of the cyclotron, a circular particle accelerator that opened the door to high-energy physics. It was Lawrence's belief that scientific research is best done through teams of individuals with different fields of expertise, working together. His teamwork concept is a Berkeley Lab legacy that continues today.