Berkeley Lab's Biological Systems & Engineering Division has an opening for a Biological Postdoctoral Scholar. The incumbent will play a key role in a collaborative project between the Joint BioEnergy Institute (JBEI, located within Berkeley Lab's Emeryville Station East [ESE4] Facility) and the Biotechnology Department within the Total oil and gas company. The project will focus on engineering a non-conventional microbe by modifying a native pathway towards the production of more valuable compounds.
The candidate will knock out chromosomal genes, overexpress native genes, and introduce heterologous genes, to obtain a strain capable of producing the desired final product. The impact of each genetic modification will be monitored and will drive the next steps to reach our target. The candidate will evaluate the bottleneck(s) and provide new solution(s) for strain improvement. You will have strong genetic and metabolic engineering skills, be comfortable with analytical and biochemical methods (e.g., enzyme production and activity assay in vivo and in vitro). You will also contribute to the preparation of periodic reports and scientific and technical peer-reviewed publications, and present results at scientific and technical meetings.
What You Will Do:
Design strains, experiments, and analytical tests to create high-performance strains.
Apply innovative genetic engineering techniques to solve metabolic bottlenecks.
Analyze scientific literature to identify and examine hypotheses to improve strain performance.
Work with researchers from various platforms: analytical, chemistry, proteomic, and bioinformatics groups to build and test engineered strains.
Conduct biochemical analysis in vitro if required to understand potential problems.
Design, modify, and optimize environmental conditions for optimal strain growth or for understanding limitation (improved media, utilization of various carbon sources, temperature, pH, anaerobic conditions, etc.).
Maintain safe practices and work environment.
Maintain records on experimental designs and results. Archive strains. Use lab computer and software to record data.
Preparation and/or contribution of periodic reports and scientific and technical peer-reviewed publications or patent applications.
Present results in technical meetings and seminars.
Additional Responsibilities as needed:
Mentor, train, and/or provide guidance to junior researchers.
Prepare media, buffers and stock solutions if needed.
Maintain clean and organized personal and common benches.
What is Required:
Ph.D. degree in Biology, Chemical Engineering or related discipline.
Demonstrated track record of generating data and results.
Prior experience in conducting presentations, writing reports/publications for scientific and technical peer-reviewed publications.
Strong knowledge in genetic engineering, metabolic engineering, and cellular processes involved in compound production, including redox balance, energetics, growth rate, and fermentation conditions.
Extensive molecular biology skills, including PCR, restriction digest analysis, design and execution of cloning experiments, DNA assembly methods, plasmid construction, DNA sequence analysis, DNA recombination, microbial transformation, and genomic DNA analyses.
Knowledge in biochemistry (protein-related in vitro assays, enzymology) and analytical skills (LC-MS).
Experience working with various organisms and especially nonconventional lab microbes.
Excellent interpersonal skills for working in a team environment.
Ability to gather feedback and recommendations towards achieving goals.
Demonstrated high productivity and strong capability of working independently and make appropriate decisions during shift work without additional support.
Ability to handle multiple tasks in parallel while working effectively.
Ability to develop or follow protocols to produce consistent reproducible results.
Ability to be flexible as well as be willing to learn new skills as appropriate.
Good organizational and record keeping skills.
Strong written and verbal communication skills.
Additional Desired Qualifications:
Experience in various software, analytical and statistic tools.
General computer application skills with software, spreadsheets, databases, and word processors.
The posting shall remain open until the position is filled.
This is a full time, 2 years, postdoctoral appointment with the possibility of renewal based upon satisfactory job performance, continuing availability of funds and ongoing operational needs. You must have less than 3 years paid postdoctoral experience. Salary for Postdoctoral positions depends on years of experience post-degree.
Full-time, M-F, exempt (monthly paid) from overtime pay.
This position is represented by a union for collective bargaining purposes.
Salary will be predetermined based on postdoctoral step rates.
This position may be subject to a background check. Any convictions will be evaluated to determine if they directly relate to the responsibilities and requirements of the position. Having a conviction history will not automatically disqualify an applicant from being considered for employment.
Work will be primarily performed at Joint BioEnergy Institute (JBEI) — Bldg. 978, 5885 Hollis St., 4th floor, Emeryville, CA.
Berkeley Lab (LBNL) addresses the world's most urgent scientific challenges by advancing sustainable energy, protecting human health, creating new materials, and revealing the origin and fate of the universe. Founded in 1931, Berkeley Lab's scientific expertise has been recognized with 13 Nobel prizes. The University of California manages Berkeley Lab for the U.S. Department of Energy's Office of Science.
Equal Employment Opportunity: Berkeley Lab is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, or protected veteran status. Berkeley Lab is in compliance with the Pay Transparency Nondiscrimination Provision under 41 CFR 60-1.4. Click here to view the poster and supplement: "Equal Employment Opportunity is the Law."
Internal Number: 85571
About Lawrence Berkeley National Laboratory
In the world of science, Lawrence Berkeley National Laboratory (Berkeley Lab) is synonymous with excellence. Thirteen scientists associated with Berkeley Lab have won the Nobel Prize. Fifty-seven Lab scientists are members of the National Academy of Sciences (NAS), one of the highest honors for a scientist in the United States. Thirteen of our scientists have won the National Medal of Science, our nation's highest award for lifetime achievement in fields of scientific research. Eighteen of our engineers have been elected to the National Academy of Engineering, and three of our scientists have been elected into the Institute of Medicine. In addition, Berkeley Lab has trained thousands of university science and engineering students who are advancing technological innovations across the nation and around the world. Berkeley Lab is a member of the national laboratory system supported by the U.S. Department of Energy through its Office of Science. It is managed by the University of California (UC) and is charged with conducting unclassified research across a wide range of scientific disciplines. Located on a 200-acre site in the hills above the UC Berkeley campus that offers spectacular... views of the San Francisco Bay, Berkeley Lab employs approximately 4,200 scientists, engineers, support staff and students. Its budget for 2011 is $735 million, with an additional $101 million in funding from the American Recovery and Reinvestment Act, for a total of $836 million. A recent study estimates the Laboratory's overall economic impact through direct, indirect and induced spending on the nine counties that make up the San Francisco Bay Area to be nearly $700 million annually. The Lab was also responsible for creating 5,600 jobs locally and 12,000 nationally. The overall economic impact on the national economy is estimated at $1.6 billion a year. Technologies developed at Berkeley Lab have generated billions of dollars in revenues, and thousands of jobs. Savings as a result of Berkeley Lab developments in lighting and windows, and other energy-efficient technologies, have also been in the billions of dollars. Berkeley Lab was founded in 1931 by Ernest Orlando Lawrence, a UC Berkeley physicist who won the 1939 Nobel Prize in physics for his invention of the cyclotron, a circular particle accelerator that opened the door to high-energy physics. It was Lawrence's belief that scientific research is best done through teams of individuals with different fields of expertise, working together. His teamwork concept is a Berkeley Lab legacy that continues today.