The Biological Systems and Engineering (BSE) Division has an opportunity for a Research Assistant position in a core facility to provide research support for an NCI-funded multi-investigator (24) and multi-institutional (20) program project that investigates structural and cell biology of DNA repair machines. The core facility provides expertise in engineering vectors for high-level expression of soluble DNA repair proteins and protein complexes, and produces high quality of protein reagents for biochemical, biophysical and structural analysis, as well as for functional characterization.
The primary tasks of this position will be performing standard laboratory protocols and routine experimental procedures using molecular biological and protein biochemical techniques under the direct supervision of the Head Scientist of the core facility. In addition, you will provide operational support for general lab function, including procurement and inventory of reagents, chemical safety and biohazard regulation compliance, maintenance of laboratory equipment, and housekeeping of the work areas. The Research Assistant will team up with other Core staff and provide critical support to ensure success of the ongoing and newly developed collaborative projects with the member laboratories of the program project.
What You Will Do:
80% - Research Support
Provide research support to the Head Scientist in performing routine molecular biological, cell biological and protein biochemical techniques and experimental procedures, including PCR, enzymatic reactions for DNA, DNA isolation and quantification, recombinant gene transfer (transformation, transfection and bacuroviral infection), recombinant protein expression and analysis, recombinant protein purification using the AKTA purification systems, DNA and protein gel electrophoresis, Western blotting, affinity pull-downs and co-immunoprecipitation, bacterial culture, and insect cell culture
Collect research data and recognize deviations from established norm
Maintain accurate and detailed records of all experimental procedures
Communicate data and results to the Head Scientist in a timely fashion for troubleshooting and experimental planning and design
Use computer software to input, process and manage data for further analysis
Generate data figures and/or tables for use in bi-weekly core meetings and technical reports
Attend and present work progress at lab meetings
Provide general assistance to other members of the research team
Ensure lab compliance with local, state and federal environmental, health and safety regulation
20% - Operational Support
Maintain laboratory work areas and proper waste disposal in accordance with LBNL chemical and biohazard safety regulations
Prepare buffers, media and solutions with accuracy and consistency
Track and order supplies and reagents to maintain stocks of commonly used items
Maintain laboratory equipment and oversee lab computer files backup
Maintain and update database of generated research reagents by the core facility
Maintain and update the chemical inventory and MSDS (material and safety data sheets)
What is Required:
Proven bench skills in molecular biology, cell biology and protein biochemistry techniques and strong working knowledge of molecular biology and protein purification instrumentation
Attention to details and strong organizational skills
Ability to follow verbal and written instructions, and work efficiently and accurately
Ability to perform and coordinate multiple experimental procedures, and prioritize assigned work
Demonstrated ability of documenting accurate and detailed procedures and results
Strong communication skills for timely report and discussion of experimental results and lab issues
Strong interpersonal skills to work and coordinate with the research team members
Familiarity with Macintosh and Windows computer systems for instrumental operation, data collection, preliminary analysis, and record keeping, including, but not limited to, Microsoft Word, Excel, and PowerPoint, Adobe Photoshop, Licor Odyssey imaging and analysis program, DNA STAR software, and FileMaker
Additional Desired Qualifications:
Bachelor's degree in biology, molecular biology or biochemistry, with relevant research experience
Experience in handling chemical and biologically hazardous materials and waste disposal
Experience in managing and updating electronic laboratory supply and reagent inventory
The posting shall remain open until the position is filled
This is a full time career appointment.
Full-time, M-F, non-exempt (hourly paid) from overtime pay.
Salary is commensurate with experience.
This position may be subject to a background check. Any convictions will be evaluated to determine if they directly relate to the responsibilities and requirements of the position. Having a conviction history will not automatically disqualify an applicant from being considered for employment.
Work will be primarily performed atWest Berkeley Biocenter (Potter St.) — Bldg. 977, 717 Potter St., Berkeley, CA.
Berkeley Lab (LBNL) addresses the world's most urgent scientific challenges by advancing sustainable energy, protecting human health, creating new materials, and revealing the origin and fate of the universe. Founded in 1931, Berkeley Lab's scientific expertise has been recognized with 13 Nobel prizes. The University of California manages Berkeley Lab for the U.S. Department of Energy's Office of Science.
Equal Employment Opportunity: Berkeley Lab is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, or protected veteran status. Berkeley Lab is in compliance with the Pay Transparency Nondiscrimination Provision under 41 CFR 60-1.4. Click here to view the poster and supplement: "Equal Employment Opportunity is the Law."
Internal Number: 84827
About Lawrence Berkeley National Laboratory
In the world of science, Lawrence Berkeley National Laboratory (Berkeley Lab) is synonymous with excellence. Thirteen scientists associated with Berkeley Lab have won the Nobel Prize. Fifty-seven Lab scientists are members of the National Academy of Sciences (NAS), one of the highest honors for a scientist in the United States. Thirteen of our scientists have won the National Medal of Science, our nation's highest award for lifetime achievement in fields of scientific research. Eighteen of our engineers have been elected to the National Academy of Engineering, and three of our scientists have been elected into the Institute of Medicine. In addition, Berkeley Lab has trained thousands of university science and engineering students who are advancing technological innovations across the nation and around the world. Berkeley Lab is a member of the national laboratory system supported by the U.S. Department of Energy through its Office of Science. It is managed by the University of California (UC) and is charged with conducting unclassified research across a wide range of scientific disciplines. Located on a 200-acre site in the hills above the UC Berkeley campus that offers spectacular... views of the San Francisco Bay, Berkeley Lab employs approximately 4,200 scientists, engineers, support staff and students. Its budget for 2011 is $735 million, with an additional $101 million in funding from the American Recovery and Reinvestment Act, for a total of $836 million. A recent study estimates the Laboratory's overall economic impact through direct, indirect and induced spending on the nine counties that make up the San Francisco Bay Area to be nearly $700 million annually. The Lab was also responsible for creating 5,600 jobs locally and 12,000 nationally. The overall economic impact on the national economy is estimated at $1.6 billion a year. Technologies developed at Berkeley Lab have generated billions of dollars in revenues, and thousands of jobs. Savings as a result of Berkeley Lab developments in lighting and windows, and other energy-efficient technologies, have also been in the billions of dollars. Berkeley Lab was founded in 1931 by Ernest Orlando Lawrence, a UC Berkeley physicist who won the 1939 Nobel Prize in physics for his invention of the cyclotron, a circular particle accelerator that opened the door to high-energy physics. It was Lawrence's belief that scientific research is best done through teams of individuals with different fields of expertise, working together. His teamwork concept is a Berkeley Lab legacy that continues today.